Video ELISA DIRECTA

Description of the Elisa technique, its sensitivity, and utility for detecting and quantifying antigens and antibodies. The direct Elisa is commonly used for antigen detection with some limitations due to the difficulty in obtaining the conjugate that recognizes the antigen.

Explanation of preparing a vaccine against a virus using recombinant DNA technology to clone and express various virus proteins. The process involves evaluating proteins equivalent to the surface antigen of the hepatitis B virus for use in direct Elisa. This includes the conjugate preparation and the necessary reactions.

Details of the Elisa procedure starting from sensitizing different antigens obtained for a study, fixing them in wells, blocking unbound antigens, and incubating to prevent interference. The plate is then washed, ensuring all gaps are filled, and the conjugate, covalently linked to peroxidase, is added to bind specifically to the antigen.

Process of detecting immunocomplexes immobilized in the wells through enzymatic reactions that produce a color change. The procedure involves using a substrate that breaks down to generate color, which indicates a positive reaction. Controls, including positive and negative samples, are necessary for result interpretation.